What is Semax?
Semax is a synthetic heptapeptide, a seven amino acid sequence supplied as a lyophilized powder for laboratory research. It is built from the 4 to 7 fragment of adrenocorticotropic hormone (ACTH), Met-Glu-His-Phe, extended at the C-terminus with the tripeptide Pro-Gly-Pro. It is handled in the laboratory as a stable, water soluble reference peptide.
Semax structure and sequence
The sequence is Met-Glu-His-Phe-Pro-Gly-Pro, molecular formula C37H51N9O10S and molecular weight 813.9 Da. It is a short linear peptide with no cysteine residues and therefore no disulfide bonds. The N-terminal methionine carries a thioether side chain that can oxidize to the sulfoxide, so the methionine sulfoxide form is one of the related substances analysts look for alongside the main peak. The proline rich C-terminal extension was added to slow enzymatic breakdown of the parent fragment, which is why the peptide also appears in stability work.
Where this material appears in the literature
The published literature on Semax includes cell culture and animal model studies in neuroscience research, alongside analytical work on its enzymatic stability. Imperial Biolab supplies the material for that kind of laboratory investigation and makes no claim about what it does in any living subject.
Related material in the catalog
Semax is listed with the other short chain peptides in the catalog, alongside Ipamorelin. The two are structurally unrelated: Semax is an ACTH fragment with a Pro-Gly-Pro extension, while Ipamorelin is a five residue synthetic peptide.
Analysis and certification
Every batch is analyzed by an independent, accredited US laboratory before release, using RP-HPLC for purity and LC-MS for identity confirmation. The certificate of analysis is published against the batch number rather than against the product page, so the document you read is the document for the vial you receive. The batch number printed on the vial label, and the QR code beside it, are the link between the two.
Purity specification
Batches are released against a ≥99% purity specification, determined by RP-HPLC peak area. A specification is a release threshold, not a description of every batch. The measured figure for the batch you receive is stated on its certificate of analysis and may sit above the specification.
Where a measured figure and the specification differ, the certificate is the record that applies to your material. Analytical figures relate to the batch they are published against and carry no implication of suitability for any purpose beyond laboratory research.
Research use only
Imperial Biolab supplies this material as a reference standard for in vitro laboratory research. It is not supplied for any use in humans or animals, it is not a drug, dietary supplement, food or cosmetic, it has not been evaluated by the FDA, and nothing on this page describes or implies any such use. Sale is restricted to persons aged 21 or over who are qualified researchers or who purchase on behalf of a research institution, laboratory or business.
| Compound |
Semax |
| Also known as |
ACTH(4-7)-Pro-Gly-Pro |
| Class |
Synthetic peptide |
| Amino acids |
7 |
| Sequence |
Met-Glu-His-Phe-Pro-Gly-Pro |
| Molecular formula |
C37H51N9O10S |
| Molecular weight |
813.9 Da |
| CAS number |
80714-61-0 |
| Appearance |
White to off white lyophilized powder |
| Purity specification |
≥99% |
| Purity method |
RP-HPLC |
| Identity method |
LC-MS |
| Solubility |
Soluble in sterile water and aqueous buffers for analytical preparation |
| Labeled quantity |
10mg per vial |
Characterization. Values above describe the compound as supplied. The figures for the batch you receive, including measured purity, are stated on its certificate of analysis.
How this material is tested
Two separate analyses are carried out on every batch before release, and both appear on the certificate.
- RP-HPLC, for purity. Reversed phase high performance liquid chromatography separates the material by hydrophobicity. The main peak is integrated against total peak area, and that ratio is the purity figure reported on the certificate.
- LC-MS, for identity. Liquid chromatography coupled to mass spectrometry confirms that the mass of the main peak matches the expected mass for the compound, which is what distinguishes the correct sequence from a closely related one.
A complete certificate carries the compound name, the batch number, the test date, the method, the measured purity and the name of the laboratory. If any of those is missing, or the batch number on the vial does not match the certificate, contact us before using the material.
Storing Semax
Stock is kept refrigerated until dispatch. Move the vial into freezer storage on arrival and keep it sealed, dry, at −20°C or below, protected from light and moisture. Returned promptly to those conditions, the dry solid is the most stable form this material takes.
Preparing stock solutions
Prepare stock solutions in sterile water or an appropriate aqueous buffer at a concentration suited to your assay, expressed in mg/mL. Allow the vial to reach room temperature before opening, add solvent down the vial wall rather than directly onto the solid, and let the material dissolve without vigorous agitation. Semax dissolves readily in water at the concentrations used for analytical work. The methionine residue is prone to oxidation, so limit the exposure of solutions to air and light.
Solutions are less stable than the lyophilized solid. Prepare close to the point of use, hold at 2 to 8°C for short periods only, and aliquot before freezing so that repeated freeze and thaw cycles are avoided.
On arrival
Check that the vial is intact and sealed, that the compound name and labeled quantity match your order, and that the batch number on the label matches the certificate published for it. If anything does not match, or the packaging has clearly failed in transit, contact us before using the material. Problems visible on arrival should be reported within 7 days of delivery, with photos of the packaging and the batch label, so that a carrier claim remains possible.
Handling and disposal
Assess the hazards of this material before handling it and use appropriate controls and protective equipment. It has not been characterized toxicologically and should be treated as potentially hazardous. Anyone handling it should be trained for the work.
Dispose of unused material, vials and packaging lawfully and in line with your institution's waste procedures.
Record keeping
Record the batch number in your notes at the point of first use, alongside the date the vial was opened and the concentration of any stock prepared. Certificates are published against the batch number rather than the product, so the batch number is the only reliable link between your results and the analytical data for the material that produced them. If a question arises about a result months later, that link is what keeps the work auditable.