What is GHK-Cu?
GHK-Cu is the copper(II) complex of the tripeptide glycyl-L-histidyl-L-lysine, supplied as a lyophilized powder for laboratory research. The tripeptide GHK was first isolated from human plasma and binds copper(II) with high affinity, and the copper complex is the form supplied here.
GHK-Cu structure and sequence
The sequence is Gly-His-Lys coordinated to a copper(II) ion, molecular formula C14H22CuN6O4 and molecular weight 401.9 Da. The free tripeptide, C14H24N6O4, has a molecular weight of 340.38 Da. Copper is held by the amino nitrogen of glycine, the deprotonated amide nitrogen of the glycine to histidine bond and an imidazole nitrogen of histidine, and that coordination gives the material its characteristic blue color.
Where this material appears in the literature
Published work on GHK-Cu is largely in vitro and preclinical, chiefly in skin and connective tissue cell models and in studies of copper peptide coordination chemistry. Imperial Biolab supplies the material for that kind of laboratory investigation and makes no claim about what it does in any living subject.
Related material in the catalog
GHK-Cu is also supplied co-lyophilized with BPC-157, TB-500 and KPV as KLOW. As a tripeptide copper complex it is the smallest molecule in that blend, and the only one that carries a bound metal ion.
Analysis and certification
Every batch is analyzed by an independent, accredited US laboratory before release, using RP-HPLC for purity and LC-MS for identity confirmation. The certificate of analysis is published against the batch number rather than against the product page, so the document you read is the document for the vial you receive. The batch number printed on the vial label, and the QR code beside it, are the link between the two.
Purity specification
Batches are released against a ≥99% purity specification, determined by RP-HPLC peak area. A specification is a release threshold, not a description of every batch. The measured figure for the batch you receive is stated on its certificate of analysis and may sit above the specification.
Where a measured figure and the specification differ, the certificate is the record that applies to your material. Analytical figures relate to the batch they are published against and carry no implication of suitability for any purpose beyond laboratory research.
Research use only
Imperial Biolab supplies this material as a reference standard for in vitro laboratory research. It is not supplied for any use in humans or animals, it is not a drug, dietary supplement, food or cosmetic, it has not been evaluated by the FDA, and nothing on this page describes or implies any such use. Sale is restricted to persons aged 21 or over who are qualified researchers or who purchase on behalf of a research institution, laboratory or business.
| Compound |
GHK-Cu |
| Also known as |
Copper tripeptide-1, glycyl-L-histidyl-L-lysine copper(II) |
| Class |
Synthetic peptide copper complex |
| Amino acids |
3 |
| Sequence |
Gly-His-Lys, copper(II) complex |
| Molecular formula |
C14H22CuN6O4 |
| Molecular weight |
401.9 Da |
| CAS number |
89030-95-5 |
| Appearance |
Blue to purple lyophilized powder |
| Purity specification |
≥99% |
| Purity method |
RP-HPLC |
| Identity method |
LC-MS |
| Solubility |
Soluble in sterile water and aqueous buffers for analytical preparation |
| Labeled quantity |
100mg per vial |
Characterization. Values above describe the compound as supplied. The figures for the batch you receive, including measured purity, are stated on its certificate of analysis.
How this material is tested
Two separate analyses are carried out on every batch before release, and both appear on the certificate.
- RP-HPLC, for purity. Reversed phase high performance liquid chromatography separates the material by hydrophobicity. The main peak is integrated against total peak area, and that ratio is the purity figure reported on the certificate.
- LC-MS, for identity. Liquid chromatography coupled to mass spectrometry confirms that the mass of the main peak matches the expected mass for the compound, which is what distinguishes the correct sequence from a closely related one.
A complete certificate carries the compound name, the batch number, the test date, the method, the measured purity and the name of the laboratory. If any of those is missing, or the batch number on the vial does not match the certificate, contact us before using the material.
Storing GHK-Cu
Stock is kept refrigerated until dispatch. Move the vial into freezer storage on arrival and keep it sealed, dry, at −20°C or below, protected from light and moisture. Returned promptly to those conditions, the dry solid is the most stable form this material takes.
Preparing stock solutions
Prepare stock solutions in sterile water or an appropriate aqueous buffer at a concentration suited to your assay, expressed in mg/mL. Allow the vial to reach room temperature before opening, add solvent down the vial wall rather than directly onto the solid, and let the material dissolve without vigorous agitation. GHK-Cu dissolves readily in water, giving a blue solution. Avoid strongly acidic conditions and chelating buffers such as EDTA, which can strip copper from the complex.
Solutions are less stable than the lyophilized solid. Prepare close to the point of use, hold at 2 to 8°C for short periods only, and aliquot before freezing so that repeated freeze and thaw cycles are avoided.
On arrival
Check that the vial is intact and sealed, that the compound name and labeled quantity match your order, and that the batch number on the label matches the certificate published for it. If anything does not match, or the packaging has clearly failed in transit, contact us before using the material. Problems visible on arrival should be reported within 7 days of delivery, with photos of the packaging and the batch label, so that a carrier claim remains possible.
Handling and disposal
Assess the hazards of this material before handling it and use appropriate controls and protective equipment. It has not been characterized toxicologically and should be treated as potentially hazardous. Anyone handling it should be trained for the work.
Dispose of unused material, vials and packaging lawfully and in line with your institution's waste procedures.
Record keeping
Record the batch number in your notes at the point of first use, alongside the date the vial was opened and the concentration of any stock prepared. Certificates are published against the batch number rather than the product, so the batch number is the only reliable link between your results and the analytical data for the material that produced them. If a question arises about a result months later, that link is what keeps the work auditable.